"If the enemy attacks Iran, our missiles will strike Tel Aviv," the official IRNA news agency quoted Khamenei's representative in the elite Revolutionary Guards, Mojhtaba Zolnoor, as saying late on Saturday.
His comments came as Iranian air defence forces were set to carry out five days of manoeuvres involving simulated attacks on the country's nuclear facilities.
The United States and its regional ally Israel have never ruled out a resort to military action to prevent Iran developing a nuclear weapon. Iran denies any such ambition.
Iran stages frequent war games and shows off new weapons in a bid to signal its readiness to counter any military action against its nuclear facilities.
