"People want to see the conviction of the heads of the sedition because they are rioters and have violated people's property and lives," the newspaper's online edition quoted lawmaker Mohammad Taghi Rahbar as saying.
"More than 150 (lawmakers) have signed two complaints," he said, referring to the first, made in October. He did not say when the present complaint was made to prosecutor Gholam Hossein Mohseni Ejie.
Rahbar said all lawmakers "except a few want a judicial confrontation with Mousavi. It is the duty of the judiciary to look into these complaints."
There are 290 deputies in parliament.
The latest complaint comes just ahead of the 31st anniversary of the Islamic revolution on Thursday, an event which is expected to turn into yet another flashpoint between opposition supporters and security forces.
Mousavi has implicitly called on his supporters, who have been opposing the re-election of President Mahmoud Ahmadinejad, to stage anti-government demonstrations.
On February 2, he issued his most scathing attack yet against the regime, saying the Iranian revolution had failed to reach its goals.
"Stifling the media, filling the prisons and brutally killing people who peacefully demand their rights in the streets indicate the roots of tyranny and dictatorship remain from the monarchist era … I don't believe that the revolution achieved its goals," he said.
Mousavi and Mehdi Karroubi, the other opposition leader, have spearheaded a campaign against Ahmadinejad since his disputed victory in the June 12 presidential poll.
Dozens of people have been killed, hundreds wounded and several put on trial since the eruption of post-election violence.
